Fig. 3. Segregation of donor and recipient sequences after sister chromatid recombination. A clone containing 7.3 and 4.0 kb XhoI–HindIII SCneo fragments was subcloned. Each subclone contains either the 4.0 or the 7.3 kb XhoI–HindIII fragment as expected for segregation of sister chromatids to daughter cells. Presumably the 4.0 kb fragment served as the donor of information during DSB repair by LTGC to generate the 7.3 kb fragment on the recipient chromatid. Genomic DNA from each subclone was digested with XhoI and HindIII and subjected to Southern blot analysis using a neo gene probe.