Fig. 2. Human ACF1 is a member of a family of WAL proteins. (A) Predicted amino acid sequence of hACF1. The WAC and WAKZ domains, PHD finger and bromodomain in hACF1 are indicated by shaded boxes. Underlined amino acids indicate tryptic peptides whose sequences were identified by ion trap tandem mass spectrometry. (B) Known human members of a family of WAL (WSTF/Acf1-like) proteins. The percentage amino acid identity between the indicated domains is shown. The human (h)WALp3 sequence was derived from the AB032254 cDNA. hWALp3 contains the TIP5 (TTF1 interaction peptide 5, EST AF000422; Jansa et al., 1998). The hWALp4 sequence was derived from AB032255 cDNA. The TIP5 peptide in hWALp3 and the N-terminal part of hWALp4 contain a putative methyl-binding domain (pMBD). (C) Phylogeny tree of the WAL proteins. Ce acf-1 is encoded by H20J04.2 cDNA, Ce wal-3 is encoded by ZK783.4 cDNA.