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. Author manuscript; available in PMC: 2012 Jul 22.
Published in final edited form as: J Mol Biol. 2011 Jul 22;410(4):653–666. doi: 10.1016/j.jmb.2011.04.063

Figure 4. Fluorescence Anisotropy competition binding assays.

Figure 4

FA competition binding assays were performed with 5 nM FITC-Sel25 and 1 μM MyrMA plus increasing concentrations of untagged Sel25, di-C6 phosphatidyl serine (PSC6) or di-C8 PI(4,5)P2 (PIP2C8). Measurements were obtained in triplicate and dissociation curves were fitted to exponential decay best fit curves.