Fig. 4.

Ler protein containing an 11 aa insertion of the H-NS linker region shows diminished oligomerization activity. Oligomerization of mutated Ler protein containing the H-NS-derived linker amino acid sequence ELLNSLAAVKS, Ler-FL08 (a), is compared with that of wild-type Ler (b). MALS experiments were performed with 250 μl injections of 0.2 mg protein ml⁻¹, as described in Fig. 3. The y axis applies to both the molecular mass standards (dotted lines) and the molecular mass calculated from MALS data (triangles).