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Oligonucleotides used in this study
| Primer | Sequence (5′ to 3′)* |
|---|---|
| 5′-hns | CCATGGGTATGAGCGAAGCACTTAAAATTCT |
| 3′-hns | CCGGAATTCCGTTGCTTGATCAGGAAATCGTC |
| K1575 | CGGGGTACCAATATGGAAACTAATTCACAT |
| K1576 | CCGGAATTCCGAATATTTTTCAGCGGTATTAT |
| proU-230 | TATCACGCAAATAATTTGTGGT |
| proU+193 | CGCCAAGCGATAGCCCAG |
| proUregF | CCGGAATTCCTTTCGCCGAATGCCGAT |
| proUregR | CGCGGATCCAGGACTGGAAGACCATCGCA |
*Restriction sites used in cloning are underlined.
