Fig. 2.

Predicted features of the CBU1224a-encoded lipoprotein involved in metal binding (LimB). In silico analyses reveal a predicted signal peptide (lower case) containing a potential lipobox (LSGC; blue box), a consensus peptide cleavage site (red line), and an N-terminal Cys (green) in mature LimB (upper case) that is predicted to be covalently modified with diacylglyceryl (DAG) and a fatty acid (FA), as observed in other Gram-negative bacteria. The uncharged Ala envelope localization residue (ELR) (circled) immediately following the N-terminal Cys suggests that mature LimB is translocated to the outer membrane, as reported for Gram-negative bacteria. Mature LimB contains ten His (red) and four Cys (orange) residues that may be involved in metal binding.