Fig. 3.

rLimB binds Ni-HRP. FW blot prepared from a SDS-PAGE gel (12.5 % acrylamide; w/v) containing separated proteins (20 μg) of whole-cell lysates of C. burnetii (mixed-cell population; lane 1), E. coli (pMAL-c5x) (2 h, no IPTG; lane 2), E. coli (pMAL-c5x) (2 h, 1 mM IPTG; lane 3), E. coli (pJB202) (2 h, no IPTG; lane 4) and E. coli (pJB202) (2 h, 1 mM IPTG; lane 5). FW blot chemiluminescent signals from native LimB and rLimB binding of Ni-HRP are indicated by closed and open arrowheads, respectively. Molecular masses were determined from protein standards.