Fig. 5.

limB expression is highest during exponential phase growth of C. burnetii in synchronous infections of cultured Vero cells. An analysis of limB transcript quantities (histogram) relative to the number of genomes (• and line) was determined by qRT-PCR and qPCR, respectively, over the course of an 8 day synchronous infection of Vero cells, as described in Methods. Values represent the means±sd from six independent determinations. Asterisks denote a statistically significant increase in limB mRNA relative to 0 days (P<0.05).