Fig. 6.

LimB is found in LCVs and SCVs and two isoforms of the protein exist. (a) FW blot prepared from an SDS-PAGE gel (12.5 % acrylamide; w/v) using whole-cell lysates of C. burnetii from a mixed-cell population (10 days) (lane 1), LCVs (5 days; lane 2) and SCVs (28 days; lane 3) (20 μg protein per lane). (b) Corresponding Western blot (W) from the other half of the gel, developed with anti-rLimB antibodies. The small (∼7.2 kDa) and large (∼8.7 kDa) isoforms of LimB are indicated by white and black arrowheads, respectively. Molecular masses were determined from pre-stained standards (Bio-Rad) and are shown in kDa.