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. 2011 Apr;157(Pt 4):966–976. doi: 10.1099/mic.0.046649-0

Fig. 7.

Fig. 7.

LimB is found in the Sarkosyl-insoluble fraction of Coxiella. (a) FW blot prepared from an SDS-PAGE gel (12.5 % acrylamide; w/v) using a mixed-cell population (lane 1), whole-cell lysates (lane 2), and Sarkosyl-soluble and Sarkosyl-insoluble fractions of the mixed-cell population (lanes 3 and 4, respectively) (20 μg protein per lane). (b) Corresponding Western blot (W) from the other half of the gel developed with anti-rLimB antibodies. Molecular masses were determined from pre-stained standards (Bio-Rad) and are shown in kDa.