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. Author manuscript; available in PMC: 2011 Jul 19.
Published in final edited form as: Mol Cell. 2007 Aug 3;27(3):435–448. doi: 10.1016/j.molcel.2007.07.015

Figure 4. PTBP2 Is a Weak Repressor of PTBP1-Regulated Exons.

Figure 4

(A) CAD cells were transfected with siRNAs against PTBP1 and/or PTBP2 as indicated, and the protein levels were analyzed by immunoblotting 72 hr posttransfection. Knockdown of both PTBP1 and PTBP2 induced a more complete derepression of (B) cassette NS exons and (C) 3′-terminal alternative exons than knockdown of PTBP1 alone. RT-qPCR in (C) was carried out as in Figure 2. Data are means of three amplifications ± SD, normalized to control siRNA transfection. For multiplex RT-PCR, we used mixtures of the corresponding F1/R1/R2 primers (see Figure 2A and Table S1).