Figure 3.

DNA damage analysis of PO and VV protein extract-treated SW480 cells. SW480 cells were treated with PO and VV protein extracts (100 μg mL⁻¹) for 24 h. After treatment, cells were fixed and then stained with propidium iodide, followed by flow cytometric analysis. Data in each panel show the percentages of cells in SubG₁ (DNA-damaged cells), G₁, S and G₂/M phases of the cell cycle. These experiments were performed at least three times and a representative experiment is presented.