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. Author manuscript; available in PMC: 2012 Jul 19.
Published in final edited form as: Biochemistry. 2011 Jun 24;50(28):6208–6216. doi: 10.1021/bi200508f

Figure 1.

Figure 1

SPR binding kinetics of the interaction between AICD and Fe65 N-term-WW-PID1-PID2 1-662 (A), Fe65 WW-PID1-PID2 236-662 (B), or Fe65 PID2 534-662 (C) performed as previously described (30). AICD was biotinylated at the N-terminus through an engineered cysteine and immobilized on a streptavidin sensor chip. Fe65 (50-500 nM) was the flowing analyte. The data were collected on a Biacore 3000 and fit using a simple 1:1 binding model yielding for Fe65 1-662 KD = 2.54 +/− 0.35, for Fe65 236-662 KD = 1.01+/−0.05, and for Fe65 534-662 KD= 0.35 +/− 0.02.