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GST LRP-CT (650 nM) was immobilized on glutathione sepharose and allowed to bind 24 μM Fe65 PID2 536-662 in the presence and absence of 650 nM AICD. GST-coupled sepharose was used as a negative control. Bound Fe65 PID2 was visualized by western blotting with anti-HIS antibody (Quiagen, Penta-His Ab). Fe65 PID2 (5 and 25 pmol) is shown as a positive control. Equal LRP-CT loading is shown by western blotting with anti-LRP antibody (11H4).
