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Fig. 2. Kinetics of Pi release from EF-G following GTP hydrolysis on the ribosome. Upper panel, short time window; lower panel, long time window. EF-G or EF-G mutants (2 mM, final concentration) were rapidly mixed with ribosomes (0.2 mM) and liberated Pi was monitored by the fluorescence of MDCC-labeled PBP (Materials and methods). 1, wild-type EF-G; 2, R59K; 3, R59M; 4, R29A.
