Fig. 5. Translocation activity of EF-G R59 mutants. (A) Multiple-turnover translocation. EF-G wt (squares), R59K (upward triangles), R59M (downward triangles), R59A (diamonds), no EF-G (circles). (B) Single-round translocation. Time courses were evaluated by single-exponential fitting with the k_app values given. 1, wild-type EF-G(4.4 s^–1); 2, R59K (0.8 s^–1); 3, R59M (0.17 s^–1); 4, R59A (0.04 s^–1). (C) Dependence of k_app of translocation on the concentration of mutant and wild-type (inset) EF-G. For k_app values measured at 2 µM factor see Table II. Symbols are as in (A). Experiments were performed as in Figure 4.