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. 2011 Jul 19;6(7):e21873. doi: 10.1371/journal.pone.0021873

Figure 5. Complementation of L. lactis knock-out strains.

Figure 5

Complementation of L. lactis knock-out strains by in trans expression of the corresponding genes from pIL252 (gray) or pIL253 (black) derivatives. Data for the uncomplemented knock-out mutants is shown in white. All strains contained plasmid pNZbcaP-GFP-H6, for nisin A-inducible expression of BcaP-GFP-H6. The growth rate (A) and the ability of the knock-out strains to produce BcaP-GFP-H6 (B) after induction with 5 ng/ml at an OD600 = 0.5 was examined at 30°C in GM17. Fluorescence values in B were normalized to the wild type, L. lactis NZ9000 (pNZbcaP-GFP-H6).