Table 5. Plasmids used in this study.
Plasmid | Description | Reference or Source |
pNZ8048 | Cmr; Expression vector with nisin A-inducible PnisA | [52] |
pNGbcaP | pNZ8048 containing bcaP downstream of PnisA | [19] |
pNGbcaP-H6 | pNZ8048 containing the bcaP-H6 gene downstream of PnisA; for expression of BcaP with 6His-tag at the C-terminus | [19] |
pNZcLIC | pNZ8048 derivative that enables cloning by the LIC-VBEx procedure | [53] |
pNZbcaP-GFP-H6 | pNZcLIC derivative containing the bcaP-GFP-H6 gene downstream of PnisA; for expression of BcaP with GFP-H6 at the C-terminus | [23] |
pNZPS1Δ9 | pNZcLIC derivative containing the PS1Δ9-H6 gene downstream of PnisA; for expression of PS1 Δ9 with 6His-tag at the C-terminus | [20] |
pNZStSUT1 | pNZcLIC derivative containing the StSut1-H6 gene downstream of PnisA; for expression of StSUT1 with His-tag at the C-terminus | [20] |
pAB0169 | Tetr; pPTL derivative carrying the promoter of llmg_0169 | [26] |
pAB2164 | Tetr; pPTL derivative carrying the promoter of llmg_2164 | [26] |
pCS1966 | Emr; oroP; 5-fluoroorotate selection/couterselection vector for chromosomal integration in L. lactis | [43] |
pCS1966::ftsH | pCS1966 derivative for deletion of chromosomal ftsH | This Work |
pCS1966::llmg_0165 | pCS1966 derivative for deletion of chromosomal llmg_0165 | This Work |
pCS1966::llmg_0169 | pCS1966 derivative for deletion of chromosomal llmg_0169 | This Work |
pCS1966::llmg_0540 | pCS1966 derivative for deletion of chromosomal oxaA2 | This Work |
pCS1966::oxaA2 | pCS1966 derivative for deletion of chromosomal llmg_1103 | This Work |
pCS1966::llmg_1115/6 | pCS1966 derivative for deletion of chromosomal llmg_1115/6 | This Work |
pCS1966::cesSR | pCS1966 derivative for deletion of chromosomal cesSR | This Work |
pCS1966::rmaB | pCS1966 derivative for deletion of chromosomal rmaB | This Work |
pCS1966::llmg_1918 | pCS1966 derivative for deletion of chromosomal llmg_1918 | This Work |
pCS1966::llmg_2163 | pCS1966 derivative for deletion of chromosomal llmg_2163 | This Work |
pVE6007 | Cmr; Replication-thermosensitive derivative of pWV01 | [54] |
pVES4196 | Emr; pORI280 derivative for deletion of chromosomal llmg_1155 | [30] |
pIL252 | Emr; low copy number derivative of pAMβ1 | [27] |
pIL252::ftsH | pIL252 derivative expressing ftsH | This Work |
pIL252::oxaA2 | pIL252 derivative expressing oxaA2 | This Work |
pIL252::cesSR | pIL252 derivative expressing cesSR | This Work |
pIL252::rmaB | pIL252 derivative expressing rmaB | This Work |
pIL252::llmg_2163 | pIL252 derivative expressing llmg_2163 | This Work |
pIL253 | Emr; high copy number derivative of pAMβ1 | [27] |
pIL253::ftsH | pIL253 derivative expressing ftsH | This Work |
pIL253::oxaA2 | pIL253 derivative expressing oxaA2 | This Work |
pIL253::cesSR | pIL253 derivative expressing cesSR | This Work |
pIL253::rmaB | pIL253 derivative expressing rmaB | This Work |
pIL253::llmg_2163 | pIL253 derivative expressing llmg_2163 | This Work |
oroP: Fluoroorotate transporter gene.
Emr: Erythromycin resistance marker.
Cmr: Chloramphenicol resistance marker.
Tetr: Tetracycline resistance marker.
PnisA: native promoter of nisA.