Table 5. Plasmids used in this study.

Plasmid	Description	Reference or Source
pNZ8048	Cmr; Expression vector with nisin A-inducible PnisA	[52]
pNGbcaP	pNZ8048 containing bcaP downstream of PnisA	[19]
pNGbcaP-H6	pNZ8048 containing the bcaP-H6 gene downstream of PnisA; for expression of BcaP with 6His-tag at the C-terminus	[19]
pNZcLIC	pNZ8048 derivative that enables cloning by the LIC-VBEx procedure	[53]
pNZbcaP-GFP-H6	pNZcLIC derivative containing the bcaP-GFP-H6 gene downstream of PnisA; for expression of BcaP with GFP-H6 at the C-terminus	[23]
pNZPS1Δ9	pNZcLIC derivative containing the PS1Δ9-H6 gene downstream of PnisA; for expression of PS1 Δ9 with 6His-tag at the C-terminus	[20]
pNZStSUT1	pNZcLIC derivative containing the StSut1-H6 gene downstream of PnisA; for expression of StSUT1 with His-tag at the C-terminus	[20]
pAB0169	Tetr; pPTL derivative carrying the promoter of llmg_0169	[26]
pAB2164	Tetr; pPTL derivative carrying the promoter of llmg_2164	[26]
pCS1966	Emr; oroP; 5-fluoroorotate selection/couterselection vector for chromosomal integration in L. lactis	[43]
pCS1966::ftsH	pCS1966 derivative for deletion of chromosomal ftsH	This Work
pCS1966::llmg_0165	pCS1966 derivative for deletion of chromosomal llmg_0165	This Work
pCS1966::llmg_0169	pCS1966 derivative for deletion of chromosomal llmg_0169	This Work
pCS1966::llmg_0540	pCS1966 derivative for deletion of chromosomal oxaA2	This Work
pCS1966::oxaA2	pCS1966 derivative for deletion of chromosomal llmg_1103	This Work
pCS1966::llmg_1115/6	pCS1966 derivative for deletion of chromosomal llmg_1115/6	This Work
pCS1966::cesSR	pCS1966 derivative for deletion of chromosomal cesSR	This Work
pCS1966::rmaB	pCS1966 derivative for deletion of chromosomal rmaB	This Work
pCS1966::llmg_1918	pCS1966 derivative for deletion of chromosomal llmg_1918	This Work
pCS1966::llmg_2163	pCS1966 derivative for deletion of chromosomal llmg_2163	This Work
pVE6007	Cmr; Replication-thermosensitive derivative of pWV01	[54]
pVES4196	Emr; pORI280 derivative for deletion of chromosomal llmg_1155	[30]
pIL252	Emr; low copy number derivative of pAMβ1	[27]
pIL252::ftsH	pIL252 derivative expressing ftsH	This Work
pIL252::oxaA2	pIL252 derivative expressing oxaA2	This Work
pIL252::cesSR	pIL252 derivative expressing cesSR	This Work
pIL252::rmaB	pIL252 derivative expressing rmaB	This Work
pIL252::llmg_2163	pIL252 derivative expressing llmg_2163	This Work
pIL253	Emr; high copy number derivative of pAMβ1	[27]
pIL253::ftsH	pIL253 derivative expressing ftsH	This Work
pIL253::oxaA2	pIL253 derivative expressing oxaA2	This Work
pIL253::cesSR	pIL253 derivative expressing cesSR	This Work
pIL253::rmaB	pIL253 derivative expressing rmaB	This Work
pIL253::llmg_2163	pIL253 derivative expressing llmg_2163	This Work

oroP: Fluoroorotate transporter gene.

Em^r: Erythromycin resistance marker.

Cm^r: Chloramphenicol resistance marker.

Tet^r: Tetracycline resistance marker.

P_nisA: native promoter of nisA.