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. 2011 Jul 19;6(7):e21728. doi: 10.1371/journal.pone.0021728

Figure 8. Electropherogram of FMR1 CGG linker PCR.

Figure 8

a) PCR product from a premutation female with 1 normal allele (Allele 1) with 30 CGG repeats and 1 premutation allele (Allele 2) with 55 CGG repeats. Using a CCG chimeric primer, AGG interruptions can be identified as loss of signal intensity spanning 5 CGG peaks, followed by restoration of signal intensity. AGG interruptions occurring in the normal allele have a loss of approximately half the CGG peak intensity due to the presence of CGG peaks from the premutation allele. Allele 1: (CGG)10-AGG-(CGG)9-AGG-(CGG)9; Allele 2: (CGG)9-AGG-(CGG)9-AGG-(CGG)35 . b) PCR product using a GGC chimeric primer and the same DNA sample as Fig. 8a. AGG interruptions result in loss of signal in 4 consecutive CGG peaks, followed by restoration in signal. Overlapping AGG interruptions results in peak intensity falling to baseline levels.