Figure 4. Effect of treatment with control and acid functionalized SWCNTs on hydrophobicity of erythrocyte membrane.

Erythrocytes (5×10⁶ /ml in PBS+1%FBS at 37°C) from Swiss (panel A) and C57Bl6 (panel B) mice were incubated with 25 µg/ml of SWCNTs and AF-SWCNTs for 1 h. Cells were washed and incubated in PBS with 4 mM ANS for 20 min in dark at 37°C, after which fluorescence spectra was determined on a fluorimeter.