Table 1. Test Quality Metrics for SPr and CD Assays for Asymmetric Self-Renewal.

Assay	% ASYM Pattern		n	p	Sen(%)	Spe(%)	PPV(%)
	Condition
	ASYM	SYM
CyA∶CyD1
SPr assay	25	3	61,60	<0.0002	44	94	88
CD assay	25	2	63,60	<0.0001	43	97	94
CyA∶Btg2
SPr assay	47	6	60,50	<10−6	58	93	90
CD assay	43	5	53,61	<10−6	64	93	88
CyA∶H2A.Z
SPr assay	43	5	58,57	<10−6	69	93	89
CD assay	37	3	63,59	<10−6	53	95	92

% ASYM Pattern, percent of evaluated sister cell pairs or sister nuclei pairs showing an asymmetric protein expression pattern; ASYM, under conditions that promote asymmetric self-renewal; SYM under conditions that promote symmetric self-renewal; n, number of pairs randomly evaluated under each condition for two independent assays; p, Fisher's exact test for the statistical confidence of specific asymmetric pattern detection vs. symmetric pattern detection; Sen, test sensitivity; Spe, test specificity; PPV, test positive predictive value.