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. 2011 Jul 19;6(7):e22129. doi: 10.1371/journal.pone.0022129

Figure 6. DKK1 Mediates GATA6 Effects on Wnt Signaling.

Figure 6

(A) Representative DKK1 and ß-catenin immunolabeling in two human pancreatic cancer tissues. Arrows in the bottom left panel indicate location of cancer cells with negative labeling for ß-catenin (all images x400) (B) Panc1, A10.7 and Hs766t cells were transfected with a shRNA against DKK1 and subjected to TOPFLASH assay. Wnt activities are presented as a ratio of OT activity to OF activity. (C and D) Panc 4.14 cells were transfected with a DKK1-expressing vector and subjected to (C) TOPFLASH assay or (D) cultured in soft agar and the number of colonies at 2 weeks counted. Panc1 cells were transfected with mock, GATA6, and/or DKK1 expressing vectors, and cells were analyzed for (E) cell proliferation, or (F) cultured in soft agar and number of colonies at two weeks counted. When appropriate, all experimental data shown represents the summary three independent experiments. *, p<0.05.