Figure 4. Enrichment of opposite effects only for cis-cis and trans-trans combinations supports a mechanistic coupling.

Inter-species differences in mRNA levels (or estimated transcription rates) and mRNA degradation were divided into the contribution of cis- and trans-mutations based on the hybrid data. The enrichment of opposite transcription and degradation effects was examined for each of the four combinations of cis and trans, by a sliding window analysis of the percentage of opposite effects as a function of the fold-changes in mRNA degradation.