Table 3. Sequences of primers used for PCR.
Gene | Gene Name | Location | Forward Primer | Reverse Primer | Temp (°C) | Cycles | Size (bp) |
SMARCC1 | SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1 | 3p21.31 | CTGGCTGGGCTGGTGGTGCAGC | TCTTCCCCAAGGTCAACTGATC | 55 | 40 | 1091 |
FKBP5 | FK506 binding protein 5 | 6p21.31 | GAGTGGGGAATGGTGAGGAAACG | CCCCTTCTGAAGTCTTCTTGCCC | 55 | 30 | 1194 |
FKBP1A | FK506 binding protein 1A | 20p13 | GGCGCACCTTCCCCAAGCGCGG | GGATGATGCCTGGGTGCCCAGT | 65 | 30 | 240 |
TRIB1 | Tribbles homolog 1 | 8q24.13 | CCCATTAAACACTACCAGGAC | TCAGGTCCCCCAGCACGATGGCT | 55 | 40 | 260 |
TRIB3 | Tribbles homolog 3 | 20p13-p12.2 | CCCCGTCCAGGAAGCCCTGGCC | CTCTCACGGTCAGCGAAGAC | 55 | 40 | 288 |
BMPR1A | Bone morphogenetic protein receptor, type IA | 10q22.3 | TGCTTCATGGCACTGGGATG | GCAGGTGGCACAGACCACAGGC | 65 | 35 | 973 |
BMPR2 | Bone morphogenetic protein receptor, type II | 2q33-q34 | TAGACTGGTGCGCCCAGGGG | AGCAGACAGGGGTTGGCCCA | 65 | 30 | 950 |
eIF2C4 | Eukaryotic translation initiation factor 2C, 4 | 1p34.3 | CGGACCTCCGGCTAGCCTGT | CCAGACCTCCCTGCCCCCTC | 65 | 30 | 550 |
eIF4EBP2 | Eukaryotic translation initiation factor 4E binding protein 2 | 10q21-q22 | GAACTCGAATCATTTATGACAG | CAACTGCATGTTTCCTGTCGTG | 55 | 40 | 189 |
ACTB | Actin-beta | 7p22 | CTGACTGACTACCTCATGAAG | ATCCACATCTGCTGGAAGGTG | 55 | 20–30 | 517 |