Fig. 2. Fkh2 affects spindle elongation and nuclear migration. Fluorescent detection of the nucleus (DAPI staining: A, C, E and G) and the mitotic spindle (indirect immunofluorescence: B, D, F and H) in the wild-type (A and B), fkh1Δ (C and D), fkh2Δ (E and F) and fkh1Δfkh2Δ (G and H) strains. Examples of defects observed: (a) multibudded cells undergoing nuclear division with a long spindle; (b) large budded cells with the nucleus at the bud neck with no apparent spindle; and (c) large budded cells with the nucleus at the bud neck with a very short spindle.