Fig. 4. Fkh2 affects the cell cycle expression of the SWI5 and CLB2 genes. (A) Northern blot analysis of RNA isolated from α-factor-synchronized cultures of the fkh1Δ and fkh2Δ strains using probes specific for the CLN2, SWI5, CLB2 and RPB4 transcripts. RNA from the 0–80 min time points of the fkh1Δ strain and the 0–90 min time points of the fkh2Δ strain, spanning approximately the first cycle following α-factor release, were analysed on the same membrane. The fkh1Δ and fkh2Δ RNA samples were collected at 10 and 15 min intervals, respectively. The midlog sample for each strain is indicated (m). The panels below the northern blots show the fold induction of the CLN2 (B), SWI5 (C) and CLB2 (D) transcripts. Samples were first quantitated relative to the RPB4 loading control, then fold induction during the cell cycle was calculated using the time 0 value for the fkh1Δ strain only. This allowed direct comparisons between the strains. The arrows indicate when maximum budding was achieved for the fkh1Δ strain (solid arrow) and the fkh2Δ strain (dotted arrow) following α-factor release.