Fig. 8. Fkh2 is required for the formation of the Mcm1–SFF complex at the ACE2 promoter. Gel mobility shift assay of extracts from wild-type yeast (W303-1a) or strains that contain deletions of the indicated combinations of forkhead genes using the wild-type ACE2 promoter fragments. The bands corresponding to the Mcm1 and Mcm1–SFF complexes are indicated.