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. 2011 Jul 19;6(7):e22488. doi: 10.1371/journal.pone.0022488

Figure 2. Henipavirus infection and antagonism of the interferon signaling pathway following treatment with exogenous Universal Interferon.

Figure 2

(A) PaLuT02 cells were treated with 1000 U of Universal Interferon and cells were harvested at 1 h intervals from 1 to 5 h. Real-time PCR was then performed for ISG54, ISG56. (B) PaLuT02 cells were infected with HeV, NiV-M and NiV-B at an MOI of 10 for 24 h pi, followed by Universal Interferon (1000 U) treatment for 3 h. Real-time PCR was then performed for ISG54, ISG56. The error bars indicate standard deviation of three independent experiments. (C) Immunofluorescent staining of duplicate infections were undertaken to determine level of virus infection using HeV P-specific antisera.