Table III. Kinetic parameters of wild-type reverse transcriptase for thymidine nucleotide analogues.

	TTP	AZT triphosphate		d4T triphosphate
		–	α-borano	–	α-borano
Km (µM)a	1.7	2.4	0.2	1.2	0.44
kcat (s–1)a	0.05	0.08	0.06	0.055	0.056
kcat/Km (µM–1s–1)	0.029	0.033	0.30	0.046	0.127
KD (µM)b	11c	7.1	7.6	21.3	18.7
kpol (s–1)b	5.4c	12.8	18.4	10.8	16
kpol/KD (µM–1s–1)	0.49c	1.8	2.4	0.51	0.85

^aA single-nucleotide incorporation and gel assay was used as described (Reardon and Miller, 1990) except that incubations were at 37°C, and the heteropolymeric DNA primer–template system as described (Canard et al., 1998). K_m and k_cat were obtained using Lineweaver–Burk or Eadie–Hofstee plots in which values of correlation coefficients were >0.98.

^bK_D and k_pol were obtained as described in Figure 5 (Kati et al., 1992). Standard deviations were <16%.

^cDeterminations carried out at 25°C and taken from Reardon and Miller (1990).