Table 2. Primers used for the amplification and sequencing of the seven loci evaluated for the B. bacilliformis MLST scheme.

locus	putative gene product	product size (bp)	position1	forward primer (5′-3′)	reverse primer (5′–3′)	reference
bvrR	regulatory protein	486	1385452–1385937	GACCGCAATATTTTGACATC	GCATCCATCAAAGCATCACGACTT	[16]
ribC	riboflavin synthase alpha subunit	349	652816–653164	GATATCGGTTGTGTTGAAGA	AAAGGCGCTAACTGTTC	[20]
ftsZ	cell division protein	497	969686–970182	CTCAAGTAGGAGTGCTGCTA	CCAATTGATCTTCCTCGTTTAC	this study
groEL	heat shock protein	442	1211811–1212252	CAACAGAAGTTGAAGTGAAAG	TAGAAATCCATTCCGCCCATT	this study
flaA	flagellin A	517	1076953–1077409	TTCACTGAAGCTGCTGATAAA	CTTGTATTTGTAACGTCGTA	this study
rnpB	RNA subunit of endoribonuclease RNase P	297	988378–988674	CGGGATCCGGGGAGGAAAGTCCGGGC	CGGAATTCRTAAGCCGGRTTCTGT	[21]
rpoB	RNA polymerase beta subunit	363	579639–580001	ACGCCTGAAGGTCCAAATAT	CTTCAGAACGGATCAATGGA	this study

¹Corresponding to the complete genome sequence of B. bacilliformis strain KC583 Genbank accession number CP000524.