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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Nat Chem Biol. 2011 Jul 3;7(8):531–537. doi: 10.1038/nchembio.598

Figure 4.

Figure 4

Characterization of the 19-residue peptide degron. (a) Alanine was substituted at seventeen positions of the 19-residue peptide fused to YFP-FKBP(F36V). Cells were stably transduced with the appropriate retrovirus and after 24 hours were treated with vehicle or 2 μM Shield-1 for 24 hours and YFP levels were scored by analytical flow cytometry. (b) Four C-terminal truncation mutants of the YFP-LID domain were prepared, and NIH3T3 cells stably transduced with these constructs were treated with either vehicle or 2 μM Shield-1 for 24 hours and evaluated by flow cytometry. Deleted residues are indicated as asterisks. (c) The 19-residue peptide and deletion mutants of this degron were fused directly to YFP to evaluate the strength of these degrons. Deleted residues are indicated as asterisks. The error bars represent the s.d. of the mean based on at least two experiments.