(A) Cell lines harboring mutant p53 were treated with 15 µM PEITC (MDA-MB-231, MDA-MB-468, SCC-4) or 10 µM PEITC (DU145, and SW480) for the indicated times. (B) Cells with wild type p53 were treated with 20 µM PEITC (HCT116, A549, and MCF-7) or 15 µM PEITC (MCF-10A) for the indicated times. (C) H1299-143A cells with p53 mutant conformation (at 37°C) are more sensitive to PEITC-induced depletion than that with wild type conformation (at 32.5°C). H1299-175H cells are used as control. Both cells were treated with 20 µM PEITC for 24 h.