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. Author manuscript; available in PMC: 2012 Jul 11.
Published in final edited form as: J Med Chem. 2011 Jan 11;54(3):809–816. doi: 10.1021/jm101199t

Figure 4. Cysteine binding in mutant (G245C) p53 DNA-binding domain by ITCs and subsequent conformational changes.

Figure 4

(A) Cysteine binding in the mutant (G245C) p53 DNA-binding domain by DMSO (control), ITCs or iodoacetamide, measured using a monochlorobimane fluorometric assay (λex 380 nm; λem 485 nm). (B) Intrinsic fluorescence emission spectra of mutant (G245C) p53 DNA-binding domain at 32°C (black solid line) incubated with, from highest intensity to lowest, DMSO (black large-dashed line), SFN (black small-dashed line), D-α-methylbenzyl ITC (dark grey line), BITC (medium grey line) or 4-phenoxybenzyl ITC (light grey line).