Table 3.
Effect of the last systemic therapy on the success rate of the initial TIL outgrowth and TIL/fragment
| Category | Number of samples | Samples with ≥40 × 106 TIL | p value* (versus no previous therapy) | Mean TIL/fragment (× 106) | p value** (versus no previous therapy) |
|---|---|---|---|---|---|
| Prior systemic therapy | |||||
| No | 69 | 49 (71)*** | -- | 15.82 | -- |
| Yes | 157 | 90 (57) | 0.06 | 11.53 | 0.02 |
| Last Systemic therapy anytime before resection | |||||
| IL-2 | 26 | 17 (65) | 0.62 | 12.49 | 0.58 |
| Other Immunotherapy | 47 | 30 (63) | 0.43 | 17.80 | 0.55 |
| Biochemotherapy | 32 | 13 (41) | 0.003 | 5.96 | 0.003 |
| Chemotherapy | 48 | 29 (60) | 0.24 | 9.43 | 0.05 |
| Targeted | 4 | 1 (25) | 0.08 | 1.17 | 0.04 |
| Impact of timing of last systemic therapy | |||||
| Day 0–30 | 43 | 20 (47) | 0.02 | 7.48 | 0.004 |
| Day 31–60 | 38 | 21 (55) | 0.14 | 11.62 | 0.06 |
| Day 61–90 | 20 | 12 (60) | 0.42 | 9.12 | 0.15 |
| >90 days | 56 | 37 (66) | 0.57 | 15.44 | 0.60 |
| Therapy within 0–30 days | |||||
| IL-2 | 7 | 4 (57) | 0.43 | 7.36 | 0.30 |
| Other Immunotherapy | 5 | 2 (40) | 0.17 | 3.08 | 0.08 |
| Biochemotherapy | 9 | 1 (11) | 0.0009 | 3.27 | 0.006 |
| Chemotherapy | 19 | 13 (68) | 1.0 | 11.8 | 0.40 |
| Targeted | 3 | 0 (0) | 0.03 | 0 | 0.03 |
*
Fisher's exact test was used. P values below 0.05 are in bold.
**
Wilcoxon rank-sum test or Kruskal-Wallis test was used.
***
Numbers in parentheses indicate percentage successful TIL growers based on total number in that group shown in the “Number of samples” column.