Table 4.

Effect of the timing of the last systemic therapy before tumor harvest on the success rate of the initial TIL outgrowth and TIL/fragment.

Category	Number of samples	Samples with ≥40 × 106 TIL	p value* (versus no previous therapy)	Mean TIL/fragment (× 106)	p value** (versus no previous therapy)
Biochemotherapy
Day 0–30	9	1 (11)***	0.0009	3.28	0.006
Day 31–60	10	2 (20)	0.003	3.10	0.006
Day 61–90	3	2 (67)	1.0	5.94	0.48
>90 days	10	8 (80)	0.72	11.26	0.77
IL-2
Day 0–30	7	4 (57)	0.43	7.36	0.30
Day 31–60	6	4 (67)	1.0	19.25	0.77
Day 61–90	4	3 (75)	1.0	7.99	0.59
>90 days	9	6 (67)	1.0	13.96	0.97
Other immunotherapy
Day 0–30	5	2 (40)	0.17	3.08	0.08
Day 31–60	8	6 (75)	1.0	26.79	0.51
Day 61–90	5	3 (60)	0.63	18.47	0.90
>90 days	29	19 (66)	0.64	17.75	0.64
Chemotherapy
Day 0–30	19	13 (68)	1.0	11.85	0.40
Day 31–60	13	8 (62)	0.52	5.84	0.06
Day 61–90	8	4 (50)	0.25	5.02	0.12
>90 days	8	4 (50)	0.25	13.94	0.71

^*Fisher's exact test was used. P values below 0.05 are in bold.

^**Wilcoxon rank-sum test or Kruskal-Wallis test was used.

^***Numbers in parentheses indicate percentage successful TIL growers based on total number in that group shown in the “Number of samples” column.