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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Shock. 2011 Aug;36(2):170–176. doi: 10.1097/SHK.0b013e3182169c76

Figure 5. AKI Induces Apoptosis in Vascular Endothelial Cells.

Figure 5

Rat Lung Microvascular Endothelial Cells (RLMVECs) were cultured in the presence of serum from animals that underwent either bilateral IRI or sham surgery. Serum was isolated 24 hours after injury; cells were then cultured in either 5%, 10% or 25% serum for 24 hours. After serum exposure, cells were stained with a FITC-conjugated antibody against Annexin V as well as PI and then evaluated by FACS. Cells exposed to IRI serum showed increased evidence of apoptosis in a dose-dependent manner (A) compared to cells exposed to sham serum. There was a statistically significant difference in apoptosis between IRI and Sham in the 10% and 25% serum groups. Notably there was no significant difference in apoptosis amongst the sham groups. (B) An RLMVEC stained with Annexin V and Hoechst for nuclear visualization reveals membrane localization of Annexin V as well as nuclear changes consistent with apoptosis.