Fig. 5. (A) Southern blot analysis of genomic DNA from wild-type and two mutant alleles of the ram-5 gene, bx30 and bx81. Four EcoRI fragments spanning the ram-5 genomic region (arrows) were used as a probe to reveal the genomic aberration in the mutant animals. EI, EcoRI. (B) Northern blot analysis of wild-type and bx81 allele. A single ram-5 transcript was detected in RNA extracted from wild-type animals but not in the sample from a bx81-carrying strain. Ethidium bromide-stained gel showed equal loading of samples.