Table 2.
13N-Metabolite profile in rat brain after intracarotid artery injection of a bolus of [13N]ammonia into portacaval-shunted (PCS) rats
| Percent of Label in Various Compounds | |||||
|---|---|---|---|---|---|
| Treatment | Time after Injection (sec) | n | Ammonia | Glutamine | Glutamate |
| None | 5 | 5 | 46.8 ± 0.9 | 52.2 ± 0.8 | 1.0 ± 0.2 |
| None | 8 | 3 | 32.3 ± 1.6 | 66.3 ± 1.8 | 1.4 ± 0.1 |
| 14-wk PCS | 5 | 3 | 61.5 ± 0.9 | 38.0 ± 0.3 | 0.6 ± 0.3 |
| 14-wk PCS | 20 | 3 | 22.9 ± 2.5 | 72.7 ± 2.8 | 1.1 ± 0.2 |
Ammonia concentrations (μM) in control rat blood, PCS rat blood, control brain and PCS brain were 59 ± 8 (n = 19), 158 ± 31 (n = 24), 176 ± 25 (n=14), 274 ± 26 (n = 14), respectively. Modified from Cooper et al., 1985b.