Table 3.
13N-Metabolite profile in brain after intracarotid artery injection of a bolus of [13N]ammonia into urease-treated rats
| Percent of Label in Various Compounds | |||||
|---|---|---|---|---|---|
| Treatment | Time after Injection (sec) | n | Ammonia | Glutamine | Glutamate |
| None | 5 | 5 | 44.6 ± 1.5 | 54.2 ± 1.8 | 1.3 ± 0.4 |
| 8-wk PCS | 5 | 5 | 60.0 ± 2.4 | 38.5 ± 2.2 | 1.3 ± 0.2 |
| Urease | 5 | 3 | 66.3 ± 6.2 | 32.8 ± 6.0 | 1.0 ± 0.1 |
| 8-wk PCS + urease | 5 | 2 | 60.7, 69.0 | 38.2, 29.6 | 1.1, 1.4 |
Ammonia concentrations (μM) in control rat blood, blood from urease-treated rats, control brain and brains from urease-treated rats were 59 ± 8 (n = 19), 322 ± 99 (n = 7), 176 ± 25 (n=14), 424 ± 49 (n = 14), respectively. Ammonia levels in blood and brain from urease treated PCS rats were not measured. Modified from Cooper et al., 1985b.