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A) Alignment of BLUF domains with known 3D structures (7–10, 46). Conserved amino acids are shown with red backgrounds, semi-conserved ones in red letters. Stars indicate residues important in BLUF domain photochemistry and are pictured in B. B) PixD monomer and amino acids (and their H-bonds) critical in the photochemistry. The α1 helix has been removed for clarity. C) The wild type PixD decamer crystal structure (6, PDB code: 2HFN).
