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. Author manuscript; available in PMC: 2012 Feb 11.
Published in final edited form as: Oncogene. 2011 Mar 21;30(32):3522–3536. doi: 10.1038/onc.2011.70

Figure 5.

Figure 5

The MEK1/ERK1/2 and PI3K/AKT pathways drive gene expression and transformation of the mutant OSE cells. A.) Western blot of lysates prepared from mutant OSE cells cultured in media alone or with either the MEK1 inhibitor UO126 (10 □M) or the PI3K inhibitor LY294002 (10 □M) for 24 hrs. B.) Colony formation of mutant OSE cells grown in soft agar is inhibited by both UO126 and LY294002. C.) Gene expression is altered in cells cultured in media alone or with either UO126 or LY294002 for 24h. D.) TRP53 immunolabeling decreases in mutant cells cultured for 24 hours with or without 10 □M UO126 or LY294002 compared to media alone.