Fig. 7. The GnTIII Matrix.
Glycans released from protein expressed in strains transformed with GnTIII fusions were analyzed by MALDI TOF as described in Materials and Methods. For each fusion a number and color was assigned based on the level of (GlcNAc)2-(Man)5-(GlcNAc)2 detected (as a % of total glycans): - / white = none detected; 1 / yellow = 0 to10%; 2 / gold = 10 to 20%; 3 / orange = 20 to 50%. Codes for glycosylation enzymes are given in Table 2. Details on leaders are given in Table 1.