Spleens from WT sham or burn-injured mice were harvested at day 7 and splenocyte suspensions were prepared. Cells were stained with fluorescently-labeled anti-GR-1, -F4/80, and -CD11b specific antibodies. The dot plots shown in (A) represent the GR-1 and CD11b staining profiles of FACS-gated live and F4/80+ spleen cells. Cells were prepared from the blood, bone marrow, and spleens of WT sham or burn mice at day 7 post-injury and stained for cell-surface GR-1+/F4/80+/CD11b+. The combined FACS data from all mice is plotted in Figure 1B. There was a significant increase in GR-1+/F4/80+/CD11b+ blood and spleen macrophages that were harvested from burn as compared to sham mice (* p<0.05, Student’s t test). Data expressed as mean ± SEM of n=4 mice per group and are representative of 3 independent experiments.