Fig. 2. The subcellular localization of Zap1 is not affected by zinc status. Wild-type (DY1457) cells bearing the vector pYef2 and zap1 mutant (ZHY6) cells bearing pMyc-Zap11–880 were grown to exponential phase in LZM-galactose supplemented with either 5 µM (–Zn) or 1000 µM (+Zn) ZnCl2. Cells were viewed by Nomarski optics or epifluorescence. DAPI was used to stain the nucleus and the myc-Zap1 protein was detected by indirect immunofluorescence. The blue fluorescence of DAPI staining was converted to red and the DAPI and myc-Zap1 images were overlaid using Adobe Photoshop (merge). Yellow color in the merged images indicates colocalization of the markers.