Fig. 1. (A) Amino acid alignments of V.alginolyticus PomB and R.sphaeroides MotB. White letters in black boxes and arrowheads show identical residues and the junction site of chimeric protein, respectively. Gray bars indicate putative transmembrane regions. (B) Restriction maps of plasmids. White boxes and the direction of solid arrowheads in the boxes indicate the vector part of pSU41 and the direction of transcription from the lac promoter, respectively. Inserted fragments from V.alginolyticus and R.sphaeroides, respectively, are indicated by a black or gray bold line. The hatched part of pRED1 is the region of the native R.sphaeroides promoter. The open arrows show the coding regions of pomA, pomB, motA and motB. VaPomB, V.alginolyticus PomB; RsMotB, R.sphaeroides MotB; H, HindIII; Xb, XbaI; B, BamHI; Sc, SacI; E, EcoRI; Sp, SpeI; Sh, SphI; X, XhoI; Sm, SmaI; Sl, SalI.