Table I. Characterization of chimeric proteins.
Salt (300 mM) | pH | CCCP (µM) | Swimming speed (µm/s) |
|||||||
---|---|---|---|---|---|---|---|---|---|---|
PomA+ |
MotA+ |
MotA +PomB (pYA7032) | YM19 | |||||||
PomAB (pYA303) | MomB5 | MomB6 | MomB3 | MomB5 | MomB6 | |||||
Na+ | 8.5 | 0 | 80 (3.8)a | 11 (0.9) | 70 (4.7) | 20 (2.3) | 26 (2.5) | 53 (3.9) | 28 (2.0) | 20 (1.8) |
20 | 64 (6.9) | 8 (1.3) | 51 (4.8) | 14 (2.0) | 20 (1.9) | 42 (3.6) | 27 (1.7) | 0 | ||
7.5 | 0 | 80 (3.7) | 10 (1.0) | 67 (7.9) | 23 (2.2) | 31 (5.5) | 54 (1.7) | 28 (1.7) | 27 (1.4) | |
20 | 10 (1.7) | 3 (1.5) | 6 (0.9) | 3 (1.7) | 5 (1.0) | 5 (2.2) | 9.9 (1.5) | 0 | ||
K+ | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 22 (2.4) | |
Li+ | 0 | 35 (4.5) | 24 (2.9) | 8.5 (1.2) | L.F.b | 5.5 (0.7) | L.F. | 4.4 (0.8) | 24 (2.2) |
aSD is shown in parentheses.
bL.F., low motile fraction.