Table II. Bacterial strains and plasmids.
| Strain or plasmid | Genotype or description | Reference or source |
|---|---|---|
| V.alginolyticus strains | ||
| VIO5 | VIK4 laf (Rifr Pof+ Laf–) | Okunishi et al. (1996) |
| VIO586 | VIO5 pomA (Rifr Laf– Pom–) | Asai et al. (1997) |
| NMB191 | VIO5 ΔpomAB (0.94 bp DraI–HpaI fragment deletion) (Rifr Laf– Pom–) | Yorimitsu et al. (1999) |
| YM19 | 138-2 pof (Pof–) | Kawagishi et al. (1995) |
| E.coli strains | ||
| DH5α | F– λ–recA1 hsdR17 endA1 supE44 thi-1 relA1 gyrA96 Δ(argF-lacZYA)U169 φ80dlacZΔM15 | Grant et al. (1990) |
| Plasmids | ||
| pSU41 | kan (Kmr) PlaclacZ α | Bertolome et al. (1991) |
| pRED1 | pUC19 2.5 kb SphI–SalI fragment (motAB+) | Shah and Sockett (1995) |
| pYA303 | pSU41 1.9 kb BamHI–SacI fragment (pomAB+) | Kojima et al. (1999) |
| pYA3031 | pSU41 0.8 kb BamHI–SpeI fragment (pomA+) and 1.1 kb SpeI–SacI fragment (pomB+) | this work |
| pYA3032 | pSU41 0.8 kb BamHI–SpeI fragment (pomA+) and 1.1 kb SpeI–SacI fragment (motB+) | this work |
| pYA701 | pSU41 0.8 kb BamHI–EcoRI fragment (motA+) | Asai et al. (1999) |
| pYA703 | pSU41 1.9 kb BamHI–SacI fragment (motAB+) | this work |
| pYA7031 | pSU41 0.8 kb BamHI–SpeI fragment (motA+) and 1.1 kb SpeI–SacI fragment (motB+) | this work |
| pYA7032 | pSU41 0.8 kb BamHI–SpeI fragment (pomA+) and 1.1 kb SpeI–SacI fragment (pomB+) | this work |
Kmr, kanamycin resistant; Mpar, motility resistant to phenamil; Rifr, rifampicin resistant; Plac, lac promoter.