Table I. Recombination stimulated by inverted Alus in wild-type, msh2 and msh6 strains and effect of sequence divergence.
| Orientation of Alus in LYS2 gene | Homology (%) | Recombination rate (×107) |
||
|---|---|---|---|---|
| Wild type | msh2 | msh6 | ||
| Direct | 100 | 3.6 (2.7–4.5) | 5.4 (3.1–7.2) | 4.0 (2.5–6.7) |
| Inverted | 100 | 6331 (4658–9659) | 1467 (1165–2062) | 6329 (5387–8609) |
| 99.7 (1 mismatch, 3′ end) | 5896 (4203–10390) | 1249 (959–1709) | 5902 (4193–9567) | |
| 99.7 (1 mismatch, 5′ end) | 2311 (1910–3513) | 636 (531–787) | 2030 (1564–3262) | |
| 99.1 (3 mismatches) | 1897 (1523–3253) | 735 (598–1100) | 1411 (1022–3802) | |
| 94 | 634 (417–1402) | 295 (210–595) | 679 (501–1099) | |
| 86 | 29 (25–33) | 17 (15–23) | 20 (19–29) | |
| 75 | 4.6 (4.0–7.6) | 7.8 (4.9–8.5) | 4.8 (3.5–8.1) | |
The inverted and direct Alu repeats were separated by 12 bp. Single mismatches at the 3′ and 5′ ends of the Alu were, respectively, distal and proximal to the spacer region of the IRs (Figure 1). Median rates and 95% confidence intervals (in parentheses) were calculated as stated in Materials and methods.