Table 1.

QPCR primers

Gene	Probe name		Sequence 5′–3′	R 2	Efficiency (%)	Amplicon size (bp)
CAMP a	all_v2.0.1631.C1	Forward	ATTGCAATTTCACCCTGAGC	0.999	94	118
		Reverse	CCAGACCTGCTCCTTCTCAC
GmSCYA123 a	EX175275_probe3	Forward	CTCAAACCTCTGCATCGTCA	0.997	96	188
		Reverse	CACGGAGAGGTAAGCAGCTC
HAMP a	all_v2.0.796.C2	Forward	CCACAGGCTCCTCTCAAGTC	0.988	89	146
		Reverse	CTGCAACTGCAATGCTGAAT
IL8 a	all_v2.0.37.C2	Forward	CCAATCTGACGGCTCTCTGT	0.990	103	116
		Reverse	ATCGGCTCCCTACTGGTTCT
C1S	sb_gmnlsfas_0002o02.t7	Forward	TCACTGACCGTTGAGACTCG	0.999	100	134
		Reverse	CCATTGTTGGAGCCGTAGTT
CEBPB2	all_v2.0.3759.C1	Forward	ACTCCGCAACCTGCTCTCT	0.995	99	183
		Reverse	GGTGATGGCTGTTTTCACCT
CTSL	all_v2.0.8187.C1	Forward	CTACGAGGCTGAGGATGGAC	0.999	99	109
		Reverse	GGCCTTCTGTAGGGCTTTCT
SDF1	all_v2.0.9962.C1	Forward	GCGCAGACATGGATATGAAA	0.999	95	115
		Reverse	GAGCGGCAGTAGCATCTCTC
all_v2.0.2958.C1	all_v2.0.2958.C1	Forward	CCCAAAAAGGTCTTTGGTCA	0.989	105	140
		Reverse	CTCCCCAAGCACATTTAACC
all_v2.0.6615.C2	all_v2.0.6615.C2	Forward	ACGCTGGGAAAATGAATCAC	0.993	89	176
		Reverse	TGAAACCTTATTGCCGGTTC
BPI/LBP	all_v2.0.992.C2	Forward	TCGGATCCCTTAGCAACATC	0.998	100	134
		Reverse	GATTTGCAGCTGGGTGTTTT
IIGP_b	all_v2.0.3462.C1	Forward	ACGACACAATGAGTGCCAAG	0.999	97	126
		Reverse	TTCCTGCTCCACACTCTCCT
CYBB	all_v2.0.1278.C1	Forward	TTGTGTAGCGTGTCGTTGAA	0.995	94	131
		Reverse	GGAAACTGAAACGCAAAAGC
GmSCYA104	EX731124_probe1	Forward	GATCCGAGGGTCACTGAATG	0.991	90	104
		Reverse	GCATTGACGCAGGGATAGTT
IIGP_a	all_v2.0.13188.C1	Forward	TACTGGTGTGGTGGAAACGA	0.998	96	106
		Reverse	TGAGAATGTGGTGGTTCCAA
18Sb		Forward	ATGGCCGTTCTTAGTTGGTG	0.990	109	180
		Reverse	GGACATTTAAGGGCGTCTCA

Genes in bold were shown to be Asal-responsive in SSH analysis (Feng et al. 2009). Genes in italics were chosen from the less stringent (FDR cutoff 0.05) gene list (ESM Table S2)

^aPrimers for these genes were designed and tested previously (Feng et al. 2009)

^bPrimers for 18S ribosomal RNA were designed and tested previously (Rise et al. 2008)